GetPrimer   An automatic GEnetic Targeting PRIMERS designer for yeasts
Targeted genome
Choose a pre-built genome assembly & annotation:

Annotation GFF style
NCBI
Ensembl

Or specify a customized genome assembly & annotation:



Plasmid

All gene

Product size and primer location

bp.
bp.
bp.
bp.
bp upstream of the insertion site.
bp upstream of the insertion site.
bp downstream of the insertion site.
bp downstream of the insertion site.
bp.
bp.
bp. bp.

Specificity
GetPrimers considers computated primers with ≥ total mismatches with off-target genomic regions as primers having on-target specificity.
For computated primers that have ≥ total mismatches with off-target genomic regions, if ≥ mismatches are within the last bp at the 3' end, GetPrimers also consider the corresponding primers having on-target specificity.

Dimer
GetPrimers considers pairs of primers that have > matches within the last bp at the 3' end to be dimer-prone.

The melting temperature (Tm) calculation




Other options










Presented by EvomicsLab@SYSUCC
yuejiaxing[at]gmail[dot]com